■ 基本信息
啟動(dòng)子: | GAL1 |
復(fù)制子: | 2μFI |
終止子: | CYC1 |
質(zhì)粒大小: | 5963bp |
原核抗性: | Amp |
真核抗性: | URA3 |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 酵母細(xì)胞 |
5'測(cè)序引物: | GAL1-F:ATTTTCGGTTTGTATTACTTC |
3'測(cè)序引物: | GALI-R:GTTCTTAATACTAACATAACT |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 酵母菌 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類型: |
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片段物種: |
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原核抗性: | Amp |
真核抗性: | URA3
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熒光標(biāo)記: |
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■ 質(zhì)粒簡(jiǎn)介
The pYC2/CT vector contains the CEN6/ARSH4 sequence (Sikorski and Hieter, 1989) for maintenance and replication in yeast. The CEN6/ARSH4 sequence is a 518 bp hybrid DNA fragment that contains a yeast centromere sequence (CEN) and an autonomously replicating sequence (ARS) (Sikorski and Hieter, 1989). The CEN6 sequence is derived from the CEN6 locus of yeast chromosome 6 (Panzeri and Philippsen, 1982) while the ARSH4 sequence is derived from the yeast histone H4-associated ARS (Bouton and Smith, 1986). When placed in a heterologous expression plasmid (i.e. pYC2/CT), the presence of the CEN6/ARSH4 sequence allows non-integrative centromeric maintenance and low copy number replication of the plasmid (generally 1–2 copies per cell).
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK950pYES2CT釀酒酵母質(zhì)粒.txt
